Publication

(1) Refereed paper

• Masaki, A., Miyamoto, N., Harshavardhini, S., Nagata, N., Tsuchikane, Y., Sekimoto, H., Kodama, Y., Suzuki, T., Shinomura, T. (2024) Light promotes asexual reproduction and mediates transcriptomic changes in Pediastrum duplex. J. Plant Res., https://doi.org/10.1007/s10265-024-01567-8.
• Tsuchikane, Y., Watanabe, M., Kawaguchi, Y.W., Uehara, K., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. (2024)  Diversity of genome size and chromosome number in homothallic and heterothallic strains of the Closterium peracerosum–strigosum–littorale complex (Desmidiales, Zygnematophyceae, Streptophyta). J. Phycol., http://doi.org/10.1111/jpy.13457 .
• Kawaguchi, Y., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. (2023) Extensive copy number variation explains genome size variation in the unicellular zygnematophycean alga, Closterium peracerosum-strigosum-littorale complex. Genome Biol. Evol., evad115, https://doi.org/10.1093/gbe/evad115.  
• Yamamoto, K., Matsuzaki, R., Mahakham, W., Heman, W., Sekimoto, H., Kawachi, M., Minakuchi, Y., Toyoda, A., Nozaki, H. (2023) Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox. iScience 26: 106893, doi.org/10.1016/j.isci.2023.106893.
• Sekimoto, H., Komiya, A., Tsuyuki, N., Kawai, J., Kanda, N., Ootsuki, R., Suzuki, Y., Toyoda, A., Fujiyama, A., Kasahara, M., Abe, J., Tsuchikane, Y., Nishiyama, T. (2023) A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium. New Phytol. 237: 1636-1651 doi/10.1111/nph.18662.
• Kawai, J., Kanazawa, M., Suzuki, R., Kikuchi, N., Hayakawa, Y., Sekimoto, H. (2022) Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum-strigosum-littorale complex by square-pulse electroporation. New Phytol. 233: 569-578, doi:10.1111/nph.17763
• Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T., Nozaki, H. (2021) Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system. Evolution 75: 2984-2993, doi:10.1111/evo.14306
• Rahman, H., Toda, E., Kobayashi, M., Kudo, T., Koshimizu, S., Takahara, M., Iwami, M., Watanabe, Y., Sekimoto, H., Yano, K., Okamoto, T. (2019) Expression of genes from paternal alleles in rice zygotes and involvement of OsASGR-BBML1 in initiation of zygotic development. Plant Cell Physiol. 60: 725-737, pcz030, https://doi.org/10.1093/pcp/pcz030
• Higo, A., Kawashima, T., Borg, M., Zhao, M., López-Vidriero, I., Sakayama, H., Montgomery, S. A., Sekimoto, H., Hackenberg, D., Shimamura, M., Nishiyama, T., Sakakibara, K., Tomita, Y., Togawa, T., Kunimoto, K., Osakabe, A., Suzuki, Y., Yamato, K. T., Ishizaki, K., Nishihama, R., Kohchi, T., Franco-Zorrilla, J. M., Twell, D., Berger, F., Araki, T. (2018) Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants. Nat. Commun. 9(1) 5283. DOI: 10.1038/s41467-018-07728-3.
• Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J.-T., Sekimoto, H. (2018) Identification of a new mating group and reproductive isolation in the Closterium peracerosum-strigosum-littorale complex., J. Plant Res. 131(5): 735-746, 10.1007/s10265-018-1043-8.
• Kanda, N., Ichikawa, M., Ono, A., Toyoda, A., Fujiyama, A., Abe, J., Tsuchikane, Y., Nishiyama, T., Sekimoto, H. (2017) CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex. Sci. Rep. 7: 17873.
• Yamamoto, K., Kawai-Toyooka, H., Hamaji, T., Tsuchikane, Y., Mori, T., Takahashi, F., Sekimoto, H., Ferris, P.J., Nozaki. H. (2017) Molecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids. PLOS One 12: e0180313.
• Abe, J., Hori, S., Sato, M., Sekimoto, H. (2016) Concanavalin A disrupts the release of fibrous material necessary for zygote formation of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Frontiers in Plant Science, 7: 1040.
• Abe, J., Hirano, N., Komiya, A., Kanda, N., Fujiwara, A., Hori, S., Tsuchikane, Y., Sekimoto, H. (2016). Preparation of knockdown transformants of unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Bio-protocol 6(10): e1813. http://www.bio-protocol.org/e1813
• Delaux, P-M., Radhakrishnan, G.V., Jayaraman, D., Cheema, J., Malbreil, M., Volkening, J.D., Sekimoto, H., Nishiyama, T., Melkonian, M., Pokorny, L., Rothfels, C.J., Sederoff, H.W., Stevenson, D.W., Surek, B., Zhang, Y., Sussman, M.R., Dunand, C., Morris, R.J., Roux, C., Wong, G.K-S., Oldroyd, G.E.D., Ané, J-M. (2015) Algal ancestor of land plants was pre-adapted for symbiosis. Proc. Natl. Acad. Sci. USA. 112: 13390-13395.
• Hirano, N., Marukawa, Y., Abe, J., Hashiba, S., Ichikawa, M., Tanabe, Y., Ito, M., Nishii, I., Tsuchikane, Y., Sekimoto, H. (2015) A Receptor-like kinase, related with cell wall sensor of higher plants, is required for sexual reproduction in the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol. 56: 1456-1462 (this paper was picked up in the Research Highlight section of this issue).
• Tsuchikane, Y., Nakai, A., Sekimoto, H. (2014) Detailed analyses on the parthenospore formation in Closterium moniliferum (Zygnematophyceae, Charophyta). Phycologia 53: 571-578
• Sato, M., Sakayama, H., Sato, M., Ito, M., Sekimoto, H. (2014) Characterization of sexual reproductive processes in Chara braunii (Charales, Charophyceae). Phycol. Res. 62: 214-221.
• Hiraide, R., Kawai-Toyooka, H., Hamaji, T., Matsuzaki, R., Kawafune, K., Abe, J., Sekimoto, H., Umen, J., Nozaki, H. (2013) The evolution of Male-Female sexual dimorphism predates the gender-based divergence of mating locus gene Mat3/RB. Mol. Biol. Evol. 30: 1038-1040.
• Hori, S., Sekimoto, H., Abe, J. (2012) Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum-strigosum-littorale complex (Zygnematophyceae, Charophyta). Phycol. Res. 60: 254-260.
• Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. (2012) Zygospore formation between homothallic and heterothallic strains of Closterium. Sex Plant Reprod. 25: 1-9.
• Yang, Y., Maruyama, S., Sekimoto, H., Sakayama, H., Nozaki, H. (2011) An extended phylogenetic analysis reveals ancient origin of “non-green” phosphoribulokinase genes from two lineages of “green” secondary photosynthetic eukaryotes: Euglenophyta and Chlorarachniophyta. BMC Research Notes 4: 330.
• Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Sekimoto, H. (2011) Stable nuclear transformation of Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol. 52 (9): 1676-1685.
• Tsuchikane, Y., Tsuchiya, M., Kokubun, Y., Abe, J., Sekimoto, H. (2011) Conjugation processes of Penium margaritaceum (Zygnematophyceae, Charophyta). Phycol. Res. 59: 74-82.
• Tsuchikane, Y., Kokubun, Y., Sekimoto, H. (2010) Characterization and Molecular Cloning of Conjugation-regulating Sex Pheromones in Homothallic Closterium. Plant Cell Physiol. 51: 1515-1523.
• Vannerum, K., Abe, J., Sekimoto, H., Inzé, D., Vyverman, W. (2010) Intracellular localization of an endogenous cellulose synthase of Micrasterias denticulata (Desmidiales, Chlorophyta) by means of transient genetic transformation. J. Phycol. 46: 839-845.
• Tsuchikane, Y., Sato, M., Ootaki, T., Kokubun, Y., Nozaki, H., Ito, M., Sekimoto, H. (2010) Sexual processes and phylogenetic relationships of a homothallic strain in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol. 46: 278-284.
• Yamada, T.K., Tsuchikane, Y., Wu, J.-T., Sekimoto, H., Miyaji, K., Nozaki, H. (2008) Morphology and molecular phylogeny of Eudorina sp. (Volvocaceae, Chlorophyceae) from Taiwan. Hikobia 15: 135-143.
• Tsuchikane, Y., Ito, M., Sekimoto, H. (2008) Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol. 44:1197-1203.
• Abe, J., Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H. (2008) Expression of exogenous genes under the control of endogenous HSP70 and CAB promoters in the Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol. 49: 625-632.
• Abe, J., Sakayori, K., Sekimoto, H. (2008) Effect of antibiotics on cell proliferation in the Closterium peracerosum-strigosum-littorale complex (Charophyceae, Chlorophyta). Biologia 63: 932-936.
• F. Takahashi, Y. Okabe, T. Nakada, H. Sekimoto, M. Ito, H. Kataoka, H. Nozaki (2007) Origins of the secondary plastids of Euglenophyta and Chlorarachniophyta as revealed by an analysis of the plastid-targeting nuclear-encoded gene psbO., J. Phycol. 43: 1302-1309.
• M. Imaizumi, H. Sekimoto, T. Noguchi (2007) Induction of sex pheromone secretion and observation on the secretary pathway in Closterium peracerosum-strigosum-littorale complex., Plant Sci. 173: 206-212.
• S. Akatsuka, Y. Tsuchikane, R. Fukumoto, T. Fujii, H. Sekimoto (2006) Physiological characterization of the sex pheromone protoplast-release-inducing protein from the Closterium peracerosum-strigosum-littorale complex (Charophyta)., Phycol. Res. 54: 116-121.
• Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T., Ito, M. (2006) Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophycean alga Closterium peracerosum-strigosum-littorale complex., Plant Physiol. 141: 271-279.
• Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M. (2006) Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction., J. Plant Res. 119: 105-113.
• F. Nakanishi, Y. Nagasawa, Y. Kabaya, H. Sekimoto, K. Shimomura (2005) Characterization of lucidin formation in Rubia tinctorum L., Plant Physiol. Biochem. 43: 921-928.
• Tsuchikane, Y., Ito, M., Fujii, T., Sekimoto, H. (2005) A sex pheromone, protoplast-release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium., Plant Cell Physiol. 46: 1472-1476.
• Tanabe, Y., Hasebe, M., Sekimoto, H., Nishiyama, T., Kitani, M., Henschel, K., Münster, T., Theißen, G., Nozaki, H., Ito, M. (2005) Characterization of MADS-box genes in charophycean green alga and its implication for the evolution of MADS-box genes., Proc. Natl. Acad. Sci. USA. 102: 2436-2441.
• S. Akatsuka, H. Sekimoto, H. Iwai, R. Fukumoto, T. Fujii (2003) Mucilage secretion regulated by sex pheromones in Closterium peracerosum-strigosum-littorale complex, Plant Cell Physiol. 44: 1081-1087.
• Sekimoto, H., Tanabe, Y., Takizawa, M., Ito, N., Fukumoto, R., Ito, M. (2003) Expressed sequence tags from Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, in the sexual reproduction process, DNA Res. 10: 147-153.
• R. Fukumoto, T. Fujii, H. Sekimoto (2003) Cloning of a cDNA encoding a sexual cell division-inducing pheromone from a unicellular green alga Closterium ehrenbergii, J. Phycol. 39: 931-936.
• Y. Tsuchikane, R. Fukumoto, S. Akatsuka, T. Fujii, H. Sekimoto (2003) Sex pheromones that induce sexual cell division in the Closterium peracerosum-strigosum-littorale complex (Charophyta), J. Phycol. 39: 303-309.
• H. Sekimoto (2002) Production and secretion of a biologically active Closterium sex pheromone by Saccharomyces cerevisiae, Plant Physiol. Biochem., 40: 789-794.
• R. Fukumoto, N. Dohmae, K. Takio, S. Satoh, T. Fujii, H. Sekimoto (2002) Purification and characterization of a pheromone that induces sexual cell division in the unicellular green alga Closterium ehrenbergii, Plant Physiol. Biochem., 40: 183-188.
• T. Tada, H. Sekimoto, M. Ohmori (2001) Biochemical characterization of an adenylate cyclase, CyaB1, in the cyanobacterium Anabaena sp. strain PCC 7120. J. Plant Res., 114: 387-394.
• S. Akaba, M. Seo, N. Dohmae, K. Takio, H. Sekimoto, Y. Kamiya, N. Furuya, T. Komano, T. Koshiba (1999). Production of homo- and hetero-dimeric isozymes from two aldehyde oxidase genes of Arabidopsis thaliana. J. Biochem., 126: 395-401.
• M. Rebers, T. Kaneta, H. Kawaide, S. Yamaguchi, Y.-Y. Yang, R. Imai, H. Sekimoto, Y. Kamiya (1999) Regulation of gibberellin biosynthesis genes during flower and early fruit development of tomato. Plant J., 17: 241-250.
• H. Sekimoto, R. Fukumoto, N. Dohmae, K. Takio, T. Fujii, Y. Kamiya (1998) Molecular cloning of a novel sex pheromone responsible for the release of a different sex pheromone in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 39: 1169-1175.
• H. Sekimoto, M. Seo, N. Kawakami, T. Komano, S. Desloire, S. Liotenberg, A. Marion-Poll, M. Caboche, Y. Kamiya, T. Koshiba (1998) Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana. Plant Cell Physiol., 39: 433-442.
• R. Fukumoto, T. Fujii, H. Sekimoto (1998) A newly identified chemotactic sexual pheromone from Closterium ehrenbergii. Sex. Plant Reprod., 11: 81-85.
• B. Endo, T. Fujii, Y. Kamiya, H. Sekimoto (1997) Analysis of genomic sequences encoding a sex pheromone from the Closterium peracerosum-strigosum-littorale complex. J. Plant Res., 110: 463-467.
• S. Kamachi, H. Sekimoto, N. Kondo, S. Sakai (1997) Cloning of a cDNA for a 1-aminocyclopropane-1-carboxylate synthase that is expressed during development of female flowers at the apices of Cucumis sativus L. Plant Cell Physiol., 38: 1197-1206.
• H. Sekimoto, M. Seo, N. Dohmae, K. Takio, Y. Kamiya, T. Koshiba (1997) Cloning and molecular characterization of plant aldehyde oxidase. J. Biol. Chem., 272: 15280-15285.
• R. Fukumoto, T. Fujii, H. Sekimoto (1997) Detection and evaluation of a novel sexual pheromone that induces sexual cell division of Closterium ehrenbergii (Chlorophyta). J. Phycol. 33: 441-445.
• T. Toyomasu, H. Kawaide, H. Sekimoto, C. von Numers, A. L Phillips, P. Hedden, Y. Kamiya (1997) Cloning and characterization of a cDNA encoding gibberellin 20-oxidase from rice (Oryza sativa L.) seedlings. Physiol. Plant., 99: 111-118.
• H. Sekimoto, Y. Sone, T. Fujii (1995) Biochemical, physiological and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol. 31: 609-613.
• T. Nojiri, T. Fujii, H. Sekimoto (1995) Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol. 36: 79-84.
• H. Sekimoto, Y. Sone, T. Fujii (1994) Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in the Closterium peracerosum-strigosum-littorale complex. Planta 193: 137-144.
• H. Sekimoto, Y. Sone, T. Fujii (1994) cDNA cloning of 42-kD subunit of protoplast-release-inducing protein from Closterium. Plant Physiol. 104: 1095-1096.
• H. Sekimoto, Y. Sone, T. Fujii (1994) A cDNA encoding a 19-kD subunit of protoplast-release-inducing protein from Closterium. Plant Physiol. 105: 447-447.
• H. Sekimoto, Y. Inoki, T. Fujii (1993) Detection and evaluation of an inducer of a diffusible mating pheromone of the heterothallic Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol. 34: 991-996.
• H. Sekimoto, S. Satoh, T. Fujii (1993) Analysis of binding of a biotinylated protoplast-release-inducing protein that induces release of gametic protoplasts in the Closterium peracerosum-strigosum-littorale complex. Planta 189: 468-474.
• H. Sekimoto, T. Fujii (1992) Analysis of gametic protoplast release in the Closterium peracerosum-strigosum-littorale complex (Chlorophyta). J. Phycol. 28: 615-619.
• H. Sekimoto, S. Satoh, T. Fujii (1990) Biochemical and physiological properties of a protein inducing protoplast release during conjugation in the Closterium peracerosum-strigosum-littorale complex. Planta 182: 348-354.

(2) Reviews and Proceedings

• Carrillo-Carrasco VP, Hernandez-Garcia J, Girou C, Grubor I, Keller J, Lim E, Schmidt V, Sorensen I, Vosolsobe S, Buschmann H, Delaux PM, Domozych D, Holzinger A, Nakagami H, Nishiyama T, Petrasek J, Renault H, Rensing SA, Rose JKC, Sekimoto H, Delwiche CD, Weijers D, de Vries J (2025) A roadmap to developing unified streptophyte algal model systems. Curr Biol 35 (14):R725-R738. doi:10.1016/j.cub.2025.05.023
• Ohtaka, K., Sekimoto, H. (2023) Zygnematophycean algae: possible models for cellular and evolutionary biology, Seminars in Cell & Developmental Biology, published online 13 April 2022, doi.org/10.1016/j.semcdb.2022.03.042
• Tsuchikane, Y., Sekimoto, H. (2019) The genus Closterium, a new model organism to study sexual reproduction in streptophytes, New Phytol. 221(1): 99-104, doi: 10.1111/nph.15334.
• Sekimoto, H. (2017) Sexual reproduction and sex determination in green algae, J. Plant Res. 130: 423-431.
• Sekimoto, H. (2004) Molecular characterization of a novel sex pheromone, PR-IP inducer, in Closterium peracerosum-strigosum-littorale complex, Jpn. J. Phycol. 52 (Supplement): 117-122.
• Sekimoto, H. (2000) Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae), J. Plant Res. 113: 343-352.
• Sekimoto, H., Satoh, S., Fujii, T. (1992) Biochemical and physiological properties of a gametic protoplast-release-inducing protein in Closterium. In proceedings of Second Japan-Korea Symposium on Phycology, Korean J. Phycol. 7: 121-129.

(3) Invited talk (International congress)

• Sekimoto, H. “Transformation, whole genome, mating-type determination, and RWP-RK genes in the heterothallic Closterium peracerosum-strigosum-littorale complex, a new model streptophyte alga” Workshop in Streptophyte Algae, Wageningen, the Netherlands, 15-16 May, 2023.
• Sekimoto, H., Tsuyuki, N., Komiya, A., Nishiyama, T. “The mechanism of mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in streptophytes” The SEB satellite meeting "Algal model systems on the rise: understanding and exploiting the algae to land plant transition", Seville, Spain, June 30, 2019.
• Sekimoto, H. “New insights into the sexual reproduction in unicellular zygnematophycean alga, Closterium peracerosum-strigosum-littoralecomplex” The 65^th NIBB Conference Marchantia Workshop 2017 “Renaissance of Marchantia polymorpha – the genome and beyond”, Okazaki, Aichi, December 17, 2017.
• Sekimoto, H., Hirano, N., Marukawa, Y., Tsuchikane, Y., Abe, J. “Intercellular communication in sexual reproduction of a unicellular charaphycean alga, Closterium peracerosum-strigosum-littorale complex” International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants, Nagoya, November 16, 2012.
• Abe, J., Sakayama, H., Suzuki, Y., Toyoda, A., Tsuchikane, Y., Sekimoto, H., Nishiyama, T. “Closterium and Chara genome and transcriptome analyses.” The International Plant & Animal Genome XX Conference, San Diego, CA, USA, January 14, 2012.
• Sekimoto, H. “Sexual Reproduction of a Unicellular Alga, Closterium” International Symposium of Intercellular Recognition and Allogeneic Authentication: Perspectives of Reproduction and Mechanisms Shared by Animals and Plants, Nagoya, January 14, 2010.
• Sekimoto, H. “Biologically active molecules involved in the sexual reproduction of Closterium peracerosum-strigosum-littorale complex” The Ninth international phycological congress, Tokyo, Japan, August 2009.
• Tsuchikane, Y., Sekimoto, H. “Biological speciation in Closterium (Zygnematales, Charophyceae): Reproductive isolation by sex pheromones” The Vth Asian Pacific Phycological Forum, (Wellington, New Zealand), November 2008.
• Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Fukuda, H., Demura, T.,  Ito, M. “Genes relating to the sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex, identified by microarray analysis” 4^th Asian Pacific Phycological Forum, (Bangkok, Thailand), October 2005.
• Sekimoto, H., Tsuchikane, Y., Akatsuka, A., Fukumoto, R., Fujii, T. "Sexual reproduction of Closterium peracerosum-strigosum-littorale complex: physiological, biochemical and molecular biological approaches." 3rd Asian Pacific Phycological Forum, (Tsukuba, Japan), July 2002.
• Fukumoto, R., Sekimoto, H. "Sex pheromones in Closterium." International Symposium of the Asian Pacific Phycological Assoiation (Yamagata), June 2001.
• Sekimoto, H. "Intercellular communication by proteinous pheromones during sexual reproduction of Closterium." 16th International Conference on Plant Growth Substances (Chiba), August 1998.
• Sekimoto, H., Fujii, T. "Intercellular communication by novel sex pheromones during sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale complex." Japan-French Workshop (ENS-LYON, France), November 1995.
• Sekimoto, H., Fujii, T. "Mode of induction of gametic protoplast-release by a proteinaceous sex pheromone in Closterium peracerosum-strigosum-littorale complex." JSPS-NUS Seminar (Tsukuba), August 1993.
• Sekimoto, H. Satoh, S., Fujii, T. "Biochemical and physiological properties of a gametic protoplast-release-inducing protein in Closterium." Second Japan-Korea Symposium on Phycology (Tsukuba), September 1991.